Sampling

As far as possible, it is desirable to obtain the containers for sampling in the laboratory where they are given free of charge.

If it is not possible to come to the laboratory, the containers must be prepared for themselves.

Required volume of the sample for water chemical analysis is 0.5L

The sample must be taken in an appropriate plastic or a glass bottle with a screw cap.

Don't use bottles that have had drinks with additives of color, smell or taste!

A sample is taken by filling the container to the top and delivering it to the laboratory as soon as possible, but not later than 24 hours.

To determine microbiological parameters, the sample must be taken in a sterile bag, which are given in the laboratory.

Read the instructions for microbiological sampling!

(E-coli, coliforms, enterococcus, quantity of microorganisms at 22°C and 37°C, clostridium perfrigens, as well as spores, pseudomonas aeruginosa, e.t.c)

Sample taking requires a sterile bag, that is given out in the laboratory.

Steps for sampling:

1. Wash your hands;
2. Before sampling, water tap should be disinfected with a 70% alcohol solution;
3. Open the water tap and let the water run for 2-3 minutes;
4. Take a sterile bag and prepare it for the sampling:
   1. The bag must be marked with a number (especially when multiple samples are taken)
   2. Tear the bag’s perforated top edge off;
   3. carefully open the bag by pulling the strips in the upper part of the outer edge of the bag each towards it.
   Warning: Do not touch the inside of the bag with your hands!
5. Put the sampling bag under the running water and fill the bag ~ ½ away.
   Warning: Under no circumstances do not fill the bag to the top!
6. The top edge of the bag shall be closed and secured by a rigid folding.
7. The sample bag must be placed and transported vertically.

Delivery time to the laboratory does not exceed 12-18 hours.

If the sample is delivered the following day, it must be stored in a refrigerator at night.

Steps for sampling:

1. Depth of topsoil sampling (orchards, agricultural land):
   1. Soil samples are collected in the chosen field with a homogeneous soil from several sites;
   2. For this purpose with a shovel, make a 20 cm deep hole with steep and smooth edges;
   3. Then, with a tablespoon pull along the smooth edge of the hole from the bottom to the top until the spoon is full;
   4. Discharge spoon’s content into a bucket;
   5. Repeat these steps in several places (15-20 places for 0.5-2 ha);
   6. Discharged soil in the bucket needs to be mixed thoroughly;
   7. ~0.5 kg of soil from the bucket have to be poured into a polyethylene bag for analysis;
   8. The bag must be marked with an address and a number (if multiple bags).
2. In orchard 2 samples must be taken, following the steps above:
   1. In the depth of topsoil (to ~20 cm) – see p.1;
   2. At the depth of fruit tree roots below the topsoil (~20 to ~40 cm depth).